braasch


Dr. Dwaine A. Braasch                                                       

Assistant Professor of Biochemistry and Molecular Biology


            

Education:

GVSU Grand Valley State University, B.S. in Biology, 1989.

USM University of Southern Mississippi, Ph.D. in Molecular Biology, Minor in Biochemistry, 1998.

HHMIlogo Howard Hughes Medical Institute , Post-doctoral Fellow 1999-2000.

UTSWmed University of Texas Southwestern Medical Center at Dallas, Post-doctoral Fellow 1999-2003.

Areas of Research:

  Inhibition of gene expression is a powerful approach to elucidate biochemical pathways.  Chemically modified oligonucleotides and their conjugates represent versatile and rapid tools to complement genetic knockout systems. My laboratory focuses on the design, synthesis, conjugation, and purification of a variety of oligonucleotide analogs.  Specifically, we aim to elucidate the rules governing the successful incorporation of chemical modifications manipulation and cellular delivery of chemically diverse oligonucleotide mimics such as peptide nucleic acids (PNAs), locked nucleic acids (LNAs) and small interfering RNA (siRNA) capable of specific inhibition of gene expression or gene activation in eukaryotic cell types.

    The realization of antisense and anti-gene applications for modified oligonucleotides as therapeutic agents can augment genetic models by regulating the expression of one or more genes in a temporal manner. The lab  focuses on the development of oligonucleotide analogs and novel peptide-carbohydrate moeities designed to facilitate or enhance their cellular delivery/uptake or activity.  We aim to use this approach to elucidate biochemical pathways related to cellular transformation/tumorigenesis, cell cycle, apoptosis, immune response and other cellular disease states. 

Publications

Kimura, Y. Turner, J.R., Braasch, D.A., and Buddington, R.K. Lumenal adenosine and adenosine 5'-monophosphate rapidly increase glucose transport by intact small intestine. Am J Physiol Gastrointest Liver Physiol.  December 289:G1-G8, 2005.

Paroo Z., Bollinger R. A., Braasch D.A., Richer E., Corey D. R., Antich P. P. and Mason R. P. Validating Bioluminescence Imaging as a High-throughput, Quantitative Modality for Assessing Tumor Burden. Mol Imaging. Apr;3(2):117-24, 2004.

Braasch, D.A., Paroo, Z., Constantinescu, A., Ren, G., Öz, O., Mason, R.P., and Corey, D.R., Biodistribution of Phosphodiester and Phosporothioate siRNA. Bioorg. Med. Chem. Let. 14(5):1139-1143, 2004.

Liu, Y., *Braasch, D.A., Corey, D.R., Isoform-Selective Inhibition of Gene Expression by Targeting Alternative Translation Initiation Sites. Accepted Biochemistry, 2003.

Braasch, D.A., Jensen, S., Kuar, K., Liu, Y., White, M.A. and Corey, D.R, RNA Interference in Mammalian Cells by Chemically-Modified RNA. Biochemistry, 42(26):7967-7975, 2003.

Braasch, D.A., Liu,Y. and Corey, D.R. Antisense Inhibition of Gene Expression by Oligonucleotides Incorporating Locked Nucleic Acids: Effect of mRNA Target Sequence and Chimera Design. Nucleic Acids. 30: 5160-5167, 2002.  

Kiahatsu, K., Braasch, D.A., Cansizoglu, A. and Corey, D.R. Enhanced Strand Invasion by Peptide Nucleic Acid-Peptide Conjugates: Synthetic Mimics of DNA Binding Proteins. Biochemistry. 41(37):11118-11125, 2002.

Elayadi, A. N., *Braasch, D.A. and Corey, D. R.  Superior Affinity and Inhibition of Telomerase by Locked Nucleic Acid (LNA) oligomers. Biochemistry. 6;41(31):9973-81, 2002.

Hwa Jeong Lee, Boado, R.J., Braasch, D.A., Corey, D.R. and William M. Pardridge, W.M.  Imaging Gene Expression in the Brain In Vivo In a Transgenic Mouse Model of Huntington’s Disease with an Antisense Radiopharmaceutical and Drug-Targeting Technology.  J. Nucl.Med. 43(7):948-956, 2002.

Doyle, D. F, Braasch, D.A., Jackson, L., Weiss, H. E., Boehm, M. F., Mangelsdorf D. J., Corey D. R.  Engineering Orthogonal Ligand Receptor Pairs from “Near Drugs”. Journal of the American Chemical Society. 123(46):11367-11371, 2001.

Doyle D. F., *Braasch, D.A., Simmons, C. G., Janowski, B. A. and Corey, D. R. Inhibition of Gene Expression Inside Cells by Peptide Nucleic Acids: Effect of mRNA Target Sequence, Mismatched Bases, and PNA Length. Biochemistry. 40(1):53-64, 2001.

Braasch D.A. Ellender R. D. and Middlebrooks B. L.  Cell Cycle Components and Their Potential Impact on the Development of Continuous In Vitro Penaeid Cell Replication. Methods in Cell Science. 21(4):255-61, 1999.

Braasch, D.A. and Ellender, R. D. and Smith, A.  KMED: A Two Step Process to Remove Color from Pulp Mill Wastewater. Tappi Journal. 81(7):149-156, 1998.

* Co-first authorships

Reviews

Braasch, D.A., and Corey, D.R.  Novel Antisense and Peptide Nucleic Acid Strategies for Controlling Gene Expression (Review) Biochemistry. 41(14); 4503-4510, 2002.

Braasch, D. A. and Corey, D. R.  Locked Nucleic Acid (LNA): Fine-Tuning the Recognition of DNA and RNA (Review) Chemistry & Biology. 8:1:1-7, 2001.

Book Chapters

Braasch, D.A., Nulf, C. and Corey, D.R. Current Protocols in Nucleic Acid Chemistry. Synthesis and Purification of Peptide Nucleic Acids.  John Wiley & Sons, Inc., Hoboken, NJ, 2002.

Braasch, D.A., and Corey, D.R. Current Protocols in Nucleic Acid Chemistry. Intracellular Delivery and Cellular Effects of Locked Nucleic Acids. John Wiley & Sons, Inc., Hoboken, NJ, 2002.

Braasch, D. A. and Corey, D. R. Methods in Molecular Biology Vol.208.: Peptide Nucleic Acids Methods and Protocols. Lipid-Mediated Introduction of Peptide Nucleic Acids into Cells. Humana Press, Inc., Totowa, NJ. 2002.

Braasch, D. A. and Corey, D. R.  Synthesis, Analysis, Purification and Intracellular Delivery of Peptide Nucleic Acids. Methods: A companion to Methods in Enzymology. 23:97-107, 2001.




Abstracts

Yasuhiro Kimura, Jerrold R Turner, Dwaine A Braasch, Randal Buddington. Rapid, non-genomic regulation of glucose transporters by adenosine and adenosine 5'-monophosphate disodium. Submitted. Experimantal Biology 2005(R) (April 2-6 at San Diego                         Convention Center, San Diego, CA; XXXV  International Congress of Physiological Sciences (March 31- April 5).

Yasuhiro Kimura, Jerrold R Turner, Dwaine A Braasch, Yasuo Nagata, Noboru Yoshino, Randal Buddington. Rapid, non-genomic regulation of glucose transporters by adenosine and AMP. Submitted. Experimantal Biology 2005(R) (April 2-6 at San Diego Convention Center, San Diego, CA; XXXV International Congress of Physiological Sciences (March 31- April 5).

Yasuhiro Kimura, Dwaine A. Braasch, Jerrold Turner, and Randal Buddington. Estradiol triggers rapid, non-genomic changes in glucose transport by mouse small intestine and Caco-2 cells.  Submitted. Digestive Disease Week 2005 (May 14-19 at McCormick                         Place, Chicago IL).

Communication Links

Phone: (662) 325-4021
Fax: (662) 325-8664
E-mail: db255@Ra.MsState.edu