National Chung-Hsing University
- B.S.
National Taiwan University - M.S.
Kent State University - Ph.D.
University of Oklahoma - Postdoctoral
Texas A & M University - Postdoctoral
We are currently focused on the study of expression and regulation of cotton genes involved in fiber development. Cotton fiber is a differentiated epidermal single cell originating from a developing seed. The development of the fiber is triggered by the phytohormones auxin and gibberellin (GA) and can be divided into four stages: initiation, elongation (synthesis of primary cell walls), synthesis of secondary cell walls, and maturation .We have cloned several cotton genes for proline-rich cell wall proteins, lipid transfer proteins, and R2R3-MYB proteins, and have shown that these genes are specifically expressed in fibers during the elongation stage. Fiber are single cells and don't divide in planta, however, it has been shown that multicelled fibers are formed when cotton ovules are cultured in vitro. Our recent data have also shown that the auxin/ubiquitin-proteasome proteolysis pathway may control fiber development and differentiation. We are now cloning fiber genes involved in cell cycle control and ubiquitination and study their role in fiber development and differentiation. We have also involved in dissecting fiber promoter/regulatory elements of fiber genes using a GUS (or GFP) reporter system.The fiber-specific promoters will be useful in cotton genetic engineering for fiber modification.
Ma, D.P., Tan, H., Si, Y., Creech, R.G., and Jenkins, J.N. 1995. Differential expression of a lipid transfer protein gene in cotton fiber. Biochim. Biophys. Acta 1257: 81-84.
Ma, D.P., Liu, H.C., Creech, R.G., Jenkins, J.N., and Chang, Y.F. 1997. Cloning and characterization of a cotton lipid transfer protein gene specifically expressed in fiber cells. Biochim. Biophys. Acta 1334: 111-114.
Handumrongkul, C., Ma, D.P., and Silva, J.L. 1998. Cloning and expression of a Candida guilliermondii xylose reductase gene (xyl1) in Pichia pastoris. Appl. Microbiol. Biotechnol. 49: 399-404.
Hsu, C.Y., Creech, R.G., Jenkins, J.N., and Ma, D.P. 1999. Analysis of promoter activity of cotton lipid transfer protein gene Ltp6 in transgenic tobacco plants. Plant Sci. 143: 63-70.
Liu, H.C., Creech, R. G., Jenkins, J. N. and Ma, D. P. 2000. Cloning and promoter analysis of the cotton lipid transfer protein gene Ltp3. Biochim. Biophys. Acta 1487: 106-111.
Ma, D. P., Liu, H. C. and Hsu, C. Y. 2001. Expression and regulation of lipid transfer protein genes in cotton fiber. In: Jenkins J.N. and Saha S. (eds). Genetic Improvement of Cotton: Emerging Technologies. Science Publishers, Inc. Enfield, NH, USA and Plymouth, UK. pp. 257-267.
Chang, Y.-F., Novosel, V., Chang, C.-F., Summers, B.A., Ma, D.P., Chiang, Y.-W., Acree, W.M., Chu, H.-J., Shin, S., and Lein, D.H. 2001. Experimental induction of chronic borreliosis in adult dogs exposed to Borrelia burgdorferi-infected ticks and treated with dexamethasone. Am. J. Vet. Res. 62: 1104-1112.
Tan, H., Creech, R. G., Jenkins, J. N., Chang, Y. F. and Ma, D. P. 2001. Cloning and expression analysis of two cotton (Gossypium hirsutum L.) genes encoding cell wall proline-rich proteins. DNA Sequence 12: 367-380.
Zhang, X.D., Callahan, F.E., Jenkins, J.N., Ma, D.P., Karaca, M., Saha, S., and Creech, R.G. 2002. A novel root-specific gene, MIC-3, with increased expression in nematode-resistant cotton (Gossypium hirsutum L.) after root-knot nematode infection. Biochim. Biophys. Acta 1576: 214-218.
Zhang, X.D., Jenkins, J.N., Callahan, F.E., Creech, R.G., Si, Y., McCarty , J.C., Saha, S., and Ma, D.P. 2003. Molecular cloning, differential expression, and functional characterization of a family of class I ubiquitin-conjugating enzyme (E2) genes in cotton (Gossypium). Biochim. Biophys. Acta 1625: 269-279.
Tan, H., Callahan, F.E., Zhang, X.D., Karaca, M., Saha, S., Jenkins, J.N., Creech, R.G., and Ma, D.P. 2003. Identification of resistance gene analogs in cotton (Gossypium hirsutum L.). Euphytica 134: 1-7.
Hsu, C.Y., Jenkins, J.N., Saha, S., and Ma, D.P. 2005. Transcriptional regulation of the lipid transfer protein gene Ltp3 in cotton fibers by a novel MYB protein. Plant Sci. 268: 167-181.
Callahan, F.E., Zhang, X.D., Ma, D.P., Jenkins, J.N., and Tucker, M.L. 2004. Comparison of MIC-3 protein accumulation in response to root-knot nematode infection in cotton lines displaying a range of resistance levels. J. Cotton Sci. 8: 186-190.
Phone: (662) 325-2640
Fax: (662) 325-8664
Internet:
dm1@Ra.MsState.edu
