Brooklyn College
(CUNY)- B.S. in Biology
and Chemistry, 1967
Florida State
University - M.S. in Biological Sciences, 1969
Florida State
University Ph.D. in Biological Sciences, 1972
University of
Georgia - Postdoctoral Research Fellow, 1973-74
Duke University
- NIH Postdoctoral Fellow, 1974-1978
Juan March Fellow, Spain
Max
Planck Institute for Biochemistry, Martinsried, Germany - Alexander
von Humboldt Fellow
University
of Malta Medical School - Fulbright Fellow
United States Military
Academy, West Point, NY - Visiting Professor
Research in my laboratory is centered around oxidative metabolism, halophilic organisms and chlorophyll photooxidations. For a number of years we have been studying enzymes such as superoxide dismutase, catalase and peroxidase. We have purified these enzymes from a number of sources, characterized them, isolated and sequenced the genes coding for them and we are now in the process of placing them in plants to see if they confer protection against the deleterious effects of oxidative stress conditions.
A second area of interest is that of the biochemistry of halophilic (salt requiring) enzymes. We have worked extensively with the bacterium Halobacterium salinarium, an organism that grows only at extremely high salt concentrations. We are examining in detail the mechanism whereby the enzymes and proteins isolated from this organism are stable only under high salt conditions.
A third area of research is an examination of enzymes purified from "extremophiles", organisms that grow in extreme environmental conditions such as low pH, high temperature, and high barometric pressures. We hope to characterize the pure proteins, clone the genes coding for the proteins, and express them in active form since these enzymes may have a utility in commercial areas.
Finally, we have an ongoing project in which we are examining the mechanism of chlorophyll photooxidation. We have found the light mediated peroxidative reactions may lead to a rapid bleaching of the chlorophyll molecule.
Cassidy, R.A., D.G. Burleson, A.V. Delgado, D.J. Kohler, M.L. Salin and B.A. Pruitt. 1999. The effects of heme proteins on nitric oxide levels and cell viability in isolated PMNs: a mechanism of toxicity. J. Leukocyte Biol. in press.
Salin, M.L., L.M. Alvarez, B.C. Lynn, B. Habulihaz and A.W. Fountain III. 1999. Photooxidative bleaching of chlorophyllin. Free Rad. Res. in press.
Penttilä, A., C.R. Boyle, and M.L. Salin. 1996. Active oxygen intermediates and chlorophyllin bleaching. Biochemical and Biophysical Research Communications. 226: 135-139.
Brown-Peterson, N.J., G.B. Begonia and M.L. Salin. 1995. Alterations in oxidative activity and SOD in H. halobium in response to aerobic respiratory inhibitors. Free Radical Biology and Medicine. 18:249-256.
Brown-Peterson, N.J. and M.L. Salin. 1995. Purification and characterization of a Mesohalic catalase from the halophilic bacterium Halobacterium halobium. J. Bacteriol. 177:378-384.
Brown-Peterson, N.J. and M.L. Salin. 1995. Salt stress in a halophilic bacterium: alterations in oxidative metabolism and oxy-intermediate scavenging systems. Can. J. Microbiol. 40:1057-1063.
Salin, M.L. and N.J. Brown-Peterson. 1995. Dealing with halophilic enzymes: purification of superoxide dismutase and a catalase-peroxidase in a high salt environment. in; Archaea: A Laboratory Manual, pp. 101-105.
Chen, H. P.A. Romo-Leroux and M.L. Salin. 1995. The iron containing superoxide dismutase- encoding gene from Chlamydomonas reinhardtii obtained by direct and inverse polymerase chain reactions. Gene 168: 113-116.
Salin, M.L. and M.W. Oli. 1995. Active oxygen intermediates in photosynthetic tissue: Studies on chlorophyllin photobleaching. Proceedings of US-China Symposium on Photoinhibition, Photoprotection and Crop Productivity, pp. 68-71.
Counce, P.A., M.L. Salin, Z.P. Tu, and C.C. Black, Jr. 1995. The relationship between photoprotection and crop productivity: Assessing the value of photoprotection as a criterion for improving crop productivity. Proceedings of US-China Symposium on Photoinhibition, Photoprotection and Crop Productivity, pp. 38-44.
Black, C.C., P.A. Counce, M.L. Salin, and Z.-P. Tu. 1995. Can the balance between photoprotection and photosynthesis in crops be tipped toward increased yields? Proceedings of U.S.-China Symposium on Photoinhibition, Photoprotection and Crop Productivity, pp.2-6.
BCH 8654 Intermediary Metabolism Syllabus
Phone: (662) 325-2640
Fax: (662) 325-8664
Internet:
mls1@Ra.MsState.edu
