Publications:
Gavini,
Dong,
H., Patil, P., Pulakat, L. and Nara Gavini, N. (2006) The Carboxyl Terminus of
NifK Protein is Involved in Formation of a Stable Nitrogenase Complex Under
Acidic Growth Conditions in Azotobacter vinelandii. Res.
J. Microbiol. 4:319-329.
Raja,
K., Pulakat, L. and Gavini, N. (2006) Genetic Complementation Studies of Human
Pin1 in Azotobacter vinelandii Revealed that it Requires Amino Terminus
of the NifM to Deliver PPIase Effect to the Fe-protein of Nitrogenase.
American J. Biochem. Biotech. 2:25-32.
Pulakat,
L., Rahman, S., Knowle, D. and Gavini, N. (2005) Identification of the region of
angiotensin II receptor AT2 involved in cGMP reduction. Cellular Signaling,
17:395-404.
Lahiri,
S., Pulakat, L. and Gavini, N. (2005) Functional
NifD-K fusion protein in Azotobacter vinelandii is a homodimeric complex
equivalent to the native heterotetrameric MoFe protein. Biochem. Biophys.
Res. Comm:337:677-684
Pulakat,
L., Cooper, S., Knowle, D., Mandavya, C., Bruhl, S. and Gavini
N. (2005) Liganddependent complex formation between the Angiotensin II
receptor subtype AT2 and Na+/H+ exchanger in mammalian cells. Peptides
26:863-873.
Kosaraju, P., Pulakat, L. and Gavini, N. (2004) Analysis of the genome of Azotobacter vinelandii revealed the presence of two genetically distinct group II introns. Genetica, 124:107-115.
Pulakat, L., Mandavya, C., and Gavini, N. (2004) Role of Phe308 in the seventh transmembrane domain of the AT2 receptor in ligand-binding and signaling. Biochem. Biophys. Res. Comm: 319: 1138-1143
Suh, M.H., Pulakat, L. and Gavini, N. (2003) Functional expression of a fusion-dimeric MoFe-protein of the nitrogenase in Azotobacter vinelandii. J. Biol. Chem. 278: 5353-5360.
Kumar, V., Knowles, D., Gavini, N. and Pulakat, L. (2002) Identification of the region of AT2 receptor needed for inhibition of the AT1 receptor-mediated inositol 1,4,5-triphosphate generation. FEBS Lett. 532: 379-386.
Suh, M.H., Pulakat, L. and Gavini, N. (2002) Functional expression of the FeMo-cofactor-specific biosynthetic genes nifEN as a NifE-N fusion-protein synthesizing unit in Azotobacter vinelandii.Biochem. Biophys. Res. Commun. 299: 233-240.
Pulakat, L, Lee, S-H. and Gavini, N. (2002) Genome of Azotobacter vinelandii: Counting of chromosomes by utilizing copy number of a selectable genetic marker. Genetica, 115: 147-158.
Pulakat, L., Gray, A., Johnson, J., Knowle, D., Burns, V. and Gavini, N. (2002) Role of C-terminal cytoplasmic domain of the AT2 receptor in ligand binding and signaling. FEBS Lett. 524: 73-78.
Gavini, N. and Pulakat, L. (2002) Role of the NifM in Maturation of the Fe-protein of Nitrogenase. Pp 228-232. In Nitrogen Fixation: Global perspectives, (eds: Finan, T.M., O'Brian, M.R., Layzell, D.B., Vessey, J.K. and Newton, W.) CABI publishing.
Knowles, D., Kurfis, J., Gavini, N. and Pulakat, L. (2001) Role of Asp297 of the AT2 receptor in high-affinity binding to different peptide ligands. PEPTIDES 22: 2145-2149.
Suh, M-H., L. Pulakat and N. Gavini. (2001). Isolation and Characterization of nifDK::kanamycin and Nitrogen Fixation Proficient Azotobacter vinelandii Strain, and its Implication on the Status of Multiple Chromosomes in Azotobacter. Genetica.100: 101-107.
Lei, S., L. Pulakat, M-H. Suh and N. Gavini. 2000. Identification of a second site compensatory mutation in the mutant Fe-protein that allows diazotrophic growth of Azotobacter vinelandii UW97. FEBS Lett. 478: 192-196.
Lei, S., L. Pulakat and N. Gavini. 2000. Activation of vanadium nitrogenase expression in Azotobacter vinelandii DJ54 revertant in the presence of molybdenum. FEBS Lett. 482: 149-153.
Lei, S., L. Pulakat, and N. Gavini. 1999. Genetic Analysis of the nif-Regulatory Genes by Utilizing the Yeast Two-Hybrid System Detected the Formation of NifL-NifA Complex that is Implicated in Regulated Expression of nif-Genes. J. Bacteriol. 181: 6535-6539.
Hausman, B. S., J.A. Williamson, R.P. Schreiner, L. Pulakat and N. Gavini. 1998. The rfb Genes in Azotobacter vinelandii are Arranged in a rfbFGC Gene Cluster: A Significant Deviation to the Arrangement of the rfb Genes in Enterobacteriaceae Biochem. Biophys. Res. Commun. 245: 572-582.
Lee, S-H., L. Pulakat, K. Parker and N. Gavini. 1998. Genetic analysis on the NifW by the Yeast Two-Hybrid system revealed that the NifW of Azotobacter vinelandii interacts with the NifZ to form a higher-order complex. Biochem. Biophys. Res. Commun. 244: 498-504.
Pulakat, L., E.T. Efuet and N. Gavini. 1998. Segregation pattern of kanamycin resistance marker in Azotobacter vinelandii did not show the constraints expected in a polyploid bacterium. FEMS Microbiol. Lett. 160: 247-252.
Gavini, N., B.S. Hausman, L. Pulakat, R.P. Schreiner and J.A. Williamson. 1997. Identification and mutational analysis of rfbG, the gene encoding CDP-D-Glucose-4,6-Dehydratase, isolated from free living soil bacterium Azotobacter vinelandii. Biochem. Biophys. Res. Commun. 240: 153-161.
Jock, C. A., Pulakat, Lee, S-H., and Gavini, N. (1997) Nucleotide sequence and genetic complementation analysis of lep from Azotobacter vinelandii. Biochem. Biophys. Res. Commun. 239: 393-400.
Pulakat, L., B.S. Hausman, S. Lei and N. Gavini. 1996. Nif- phenotype of Azotobacter vinelandii UW97: Characterization and mutational analysis. J. Biol. Chem. 271:1864-1869.
Lee, S-H., L. Pulakat, B.S. Hausman, E.T. Efuet, S. Lei and N. Gavini. 1996. Molecular investigations on the Dilemma of Nitrogen fixation Characteristics of Azotomonas. J. Basic. Microbiol. 36 (2):99-105.
Efuet, E. T., Pulakat, L. and Gavini, N. (1996) Investigations on the cell volumes of Azotobacter vinelandii by Scanning Electron Microscopy. J. Basic. Microbiol. 36 (4):229-234.
Gavini, N., Li. Ma, G. Watt and B.K. Burgess. 1994. Purification and characterization of a FeMoco-deficient MoFe protein of nitrogenase. Biochemistry 33:11842-11849.
Ma, Li., N. Gavini, H.I. Liu, B. Hedman, K.O. Hodgson, and B.K. Burgess. 1994. Large scale isolation and characterization of the molybdenum-iron cluster from nitrogenase. J. Biol. Chem. 269:18007-18015.
Liu, H.I., A. Filipponi, N. Gavini, B.K. Burgess, B. Hedman, A. Di Cicco, C.R. Natoli and K.O. Hodgson. 1994. EXAFS studies of FeMo-cofactor and MoFe protein: Direct evidence for the long-range Mo-Fe-Fe interaction and cyanide binding to the Mo in FeMo-cofactor. J. Amer. Chem. Soc. 116:2418-2423.
Chen, L., N. Gavini, H. Tsuruta, D. Eliezer, B.K. Burgess, S. Doniach, and K.O. Hodgson. 1993. MgATP-induced conformational changes in the iron protein from A. vinelandii, as studied by small angle x-ray scattering. J. Biol. Chem. 269:3290-3294.
Gavini, N., and B.K. Burgess. 1992. FeMoco synthesis by a nifH mutant with altered MgATP reactivity. J. Biol. Chem. 267:21179-21186.
Gavini, N., and L. Pulakat. 1992. The tRNA species for redundant genetic codons NNU and NNC: A thought on the absence of phenylalanine tRNA with AAA anticodon in Escherichia coli. J. Biol. Chem. 267:2240-2243.
Gavini, N., and L. Pulakat. 1991. Role of translation of pheA leader peptide coding region in the attenuation regulation of Escherichia coli gene pheA. J. Bacteriol. 173:4904-4907.
Gavini, N., and B. Davidson. 1991. Regulation of pheA expression by the pheR product in Escherichia coli is mediatedthrough attenuation of transcription. J. Biol. Chem. 266:7750-7753.
Gavini, N., and B. Davidson. 1990. The pheAo mutants of Escherichia coli have a defective pheA attenuator. J. Biol. Chem. 265:21532-21535.
Gavini, N. and B. Davidson. 1990. The pheR gene of Escherichia coli encodes a phenylalanyl tRNA, not a repressor protein. J. Biol. Chem. 265:21527-21531.