Ramakrishna Nannapaneni, Ph.D.   Assistant Professor Immunology & Molecular Food Microbiology Office Location: 202 Herzer Email: nannapaneni@fsnhp.msstate.edu Phone: 662-325-7697 Fax: 662-325-8728

Education

● Postdoctoral (Food Microbiology), Department of Food Science, University of Arkansas
● Ph.D. (Food Microbiology), Dept. Bioscience & Biotechnology, Univ. of Strathclyde, UK
● M.S. (Agriculture and Plant Pathology), G.B.P.U. Agriculture & Technology, India
● B.S. (Agriculture and Animal Husbandry), G.B.P.U. Agriculture & Technology, India

Professional Memberships

Member of American Society for Microbiology
Member of International Association for Food Protection
Member of Institute of Food Technologists
Member of National Alliance for Food Safety and Security

Research Mission

Newly emerging stress-hardened and antimicrobial resistant subpopulations of foodborne bacterial pathogens, Listeria monocytogenes, Vibrio, Campylobacter, Salmonella, Shigella, and E. coli O157:H7 for safety of raw, raw-further processed and ready-to-eat food products.

Research Interests

● Microbial Ecology of Fish Products
● Natural Antimicrobials and Emerging Food Processing Technologies
● Microbial Stress-Adaptation
● Antimicrobial Resistant Subpopulations
● Cultural, Immunological & Molecular Diagnostics for Viable Cells
● Integration of the “–Omics” Approaches
● Interdisciplinary Research Needs on Foodborne Bacterial Pathogens

Current Research Program

My lab’s focus is in understanding how foodborne pathogenic bacteria sense, adapt, resist and recover from different food processing stresses and antimicrobials on raw, raw-further processed and ready-to-eat food products of animal and plant origin using “whole-cell” models and “‑omics” approaches and consists of the following five objectives:

(1) Quantitative modeling of their s urvival, growth, persistence and ecological fitness of stress-hardened and antimicrobial resistant foodborne bacterial pathogens in various food products and environments.
(2) Induction of cold tolerance and subsequent interactions with other stress environments on persistence of foodborne pathogens and spoilage bacteria on fish and other food products.
(3) Inact ivation models for destruction of stress-adapted and antimicrobial resistant foodborne bacterial pathogens in various food products by combining with newly discovered antimicrobials and emerging food processing technologies.
(4) Improving methodologies for isolation, detection, and enumeration of injured, stress-hardened, viable, and antimicrobial resistant foodborne bacterial pathogen subpopulations surviving in foods and environments using a combination of cultural, immunological and molecular tools.
(5) Integration of genomic, transcriptomic, proteomic, lipomic and metabolomic data governing specific stress-response adaptation and antimicrobial resistance of foodborne bacterial pathogens and their applications to food processing industries.

Also, I have an active research program on new monoclonal antibody production for foodborne bacterial pathogens, immunodiagonstics and in developing novel therapeutic agents for Biodefense Category B priority pathogens.

Teaching Interests

● Food Microbiology
● Advanced Food Microbiology
● Aquaculture & Seafood Product Safety
● Food Safety and Biosecurity
● Distance Education in Food Safety
● Distance Education in Biosecurity

Recent Peer Reviewed Journal Articles

 Heo , S.A. , R. Nannapaneni, R. Story, and M.G. Johnson. 2007. Characterization of new hybridoma clones producing monoclonal antibodies reactive against both live and heat-killed Listeria monocytogenes. Journal of Food Science 72:M008-M015.

Wesley, I. V., S. Larsen, H. Scott Hurd, J. D. McKean, R. Griffith, F. Rivera, R. Nannapaneni, M. Cox,, M. G. Johnson, D. Wagner, and M. de Martino. 2007. The Prevalence of L. monocytogenes in cull sows. Poultry Science (submitted).

Osman, M., M.E. Janes, R. Story, R. Nannapaneni, M. G. Johnson. 2006. Differential killing activity of cetylpyridinium chloride ( CPC) with or without bacto neutralizing buffer quench, against frimly adhered Salmonella Gaminara and Shigella sonnei on cut lettuce stored at 4 C. Journal of Food Protection 69: 1286-1291.

Nannapaneni R ., R. Story, K.C. Wiggins, and M.G. Johnson. 2005. Concurrent quantitation of total Campylobacter and total ciprofloxacin-resistant Campylobacter loads in rinses from retail raw chicken carcasses from 2001 to 2003 by direct plating at 42ºC. Applied Environmental Microbiology 71:4510-4515.

Huff, G. R., W.E. Huff, J. N. Beasley, N.C. Rath, M.G. Johnson and R. Nannapaneni. 2005. Respiratory infection of turkeys with Listeria monocytogenes Scott A. Avian Diseases 49: 551-557.

Mendonca, A.F., M.G. Romero, M.A. Lihono, R. Nannapaneni and M.G. Johnson. 2004. Radiation resistance and virulence of L. monocytogenes Scott A following starvation in physiological saline. Journal of Food Protection 67: 470-474.

Zhu, M. I.V. Wesley, R. Nannapaneni, M. Cox, A. Mendonca , M.G. Johnson and D. U. Ahn. 2003. The role of vitamin E in experimental Listeria monocytogenes infections in turkeys. Poultry Science 82: 1559-1564.

Varshney, M., Y. Li, R. Nannapaneni, M.G. Johnson, C.L. Griffis. 2003. A chemiluminescence biosensor coupled with immunomagnetic separation for rapid detection of Salmonella typhimurium. Journal of Rapid Methods and Automation in Microbiology 11: 111-131.

Recent Book Chapters and Proceedings:

Nannapaneni, R. S. C. Ricke and M. G. Johnson. 2007. Campylobacter antibiotic resistance in poultry production and pathogenesis mechanisms, FSC symposium book (submitted).

Nannapaneni, R., R. Story, K.C. Wiggins, and M.G. Johnson. 2006. New monoclonal antibody probes against Campylobacter and Campylobacter jejuni, 6p, in 2006 Food Safety Consortium Annual Report, Oct. 1-3, 2006 University of Arkansas, Fayetteville.

Nannapaneni, R., K.C. Wiggins, R. Story, and M.G. Johnson. 2006. Invasiveness of six-month starvation-stressed cells of Listeria monocytogenes Scott A serotype 4b in human cell line Caco-2, 5p, in 2006 Food Safety Consortium Annual Report, Oct. 1-3, 2006 University of Arkansas, Fayetteville.

Nannapaneni, R., R. Story, K.C. Wiggins, and M.G. Johnson. 2006. Total Campylobacter and total ciprofloxacin-resistant Campylobacter loads in rinses from retail raw chicken carcasses in 2005, 5p, in 2006 Food Safety Consortium Annual Report, Oct. 1-3, 2006 University of Arkansas, Fayetteville.

Nannapaneni, R., R.Story, K.C. Wiggins, and M.G. Johnson. 2005. ELISA reactivities of newly developed monoclonal antibody producing hybridoma clones against Listeria monocytogenes and L. innocua non-starved and saline-starved cells, 5p, in 2005 Food Safety Consortium Annual Report, Oct. 2-4, 2005 Kansas State University, Manhattan.

Nannapaneni, K.C. Wiggins, R. Story and M.G. Johnson. 2005. Listeriamonocytogenes Scott A cells starved in physiological saline for one year remain highly virulent, 5p, in 2005 Food Safety Consortium Annual Report, Oct. 2-4, 2005 Kansas State University, Manhattan.

Nannapaneni, K.C. Wiggins, R. Story and M.G. Johnson. 2005. Total Campylobacter and total ciprofloxacin-resistant Campylobacter loads in rinses from retail raw chicken carcasses from 2001 to 2004, 5p, in 2005 Food Safety Consortium Annual Report, Oct. 2-4, 2005 Kansas State University, Manhattan.

Nannapaneni, K.C. Wiggins, R. Story and M.G. Johnson. 2005. Monoclonal antibody probes to Campylobacter jejuni, 5p, in 2005 Food Safety Consortium Annual Report, Oct. 2-4, 2005 Kansas State University, Manhattan.

Recent Presentations at National Meetings:

Nannapaneni, R., K.C. Wiggins, R. Story, and M.G. Johnson. 2007. Quantitative trends in total Campylobacter and total ciprofloxacin-resistant Campylobacter loads in rinses from retail raw chicken carcasses from 2001 to 2005. P-032, 107 th American Society of Microbiology Gen. Mtg. May 22-25, Ontario, Canada.

Nannapaneni, R., K.C. Wiggins, R. Story, S.C. Ricke, and M.G. Johnson. 2007. Differences in total Campylobacter and total ciprofloxacin-resistant Campylobacter loads on retail raw chicken carcasses from one processor but two different retail sources in 2006. P-038, 107 th American Society of Microbiology Gen. Mtg. May 22-25, Ontario, Canada.

Nannapaneni, R., E. Friedly, P.G. Crandall, S.C. Ricke, and M.G. Johnson. 2007. Campylobacter jejuni and C. coli sensitivity to citrus-based natural extracts. P-052, 107 th American Society of Microbilogy Gen. Mtg., May 22-25, Toronto, Canada.

Nannapaneni, R., P.G. Crandall, S.C. Ricke, and M.G. Johnson. 2007. C iprofloxacin-sensitive and ciprofloxacin-resistant Campylobacter jejuni inhibition by citrus-based natural extracts. P4-45, International Association for Food Protection, July 8-11, 2007, Lake Buena Vista, Florida.

Nannapaneni, R., P.G. Crandall, S.C. Ricke, and M.G. Johnson. 2007. Antimicrobial activity of citrus-based natural extracts against Escherichia coli O157:H7. P4-44, International Association for Food Protection, July 8-11, 2007, Lake Buena Vista, Florida.

Nannapaneni, R., Keith C. Wiggins, Robert Story, Aubrey F. Mendonca, and Michael G. Johnson. 2006. Invasiveness of six-months starvation-stressed cells of Listeria monocytogenes ScottA serotype 4b in human cell line Caco-2. P-053, Amer Soc Microbiology Gen Mtg., May 21-25, 2006, Orlando, FL.

Nannapaneni, R., Keith C. Wiggins, Robert Story, Aubrey F. Mendonca, and Michael G. Johnson. 2006. One-year starvation-stressed cells of Listeria monocytogenes ScottA serotype 4b invade human cell line Caco-2. P-04 – 53, International Association for Food Protection, Aug 13-16, 2006, Calgary, Alberta, Canada.

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Last updated: September 7, 2007
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